<i>In vitro</i> experience of human natural killer cell culture with feeder cells

Adoptive immunotherapy using NK cells has become a promising therapeutic area. are component of the innate immune system, act as key regulators, and have potent antitumor cytolytic activity. In order to be able evaluate effect adoptive cell at preclinical stages, there is need for reliable protocols in vitro production cells. There large number publications on methods activating generating human cells, including feeder-cells various cytokines. The article describes experience cultivation from cancer patients or donors with feedercells without (control group). K562 line was used feeder after irradiation two types: gene modification (gmK562) membrane-bound mbIL15, mbIL21 it. were mixed ratio 1:1, 1:2 1:5 0, 7 14 days respectively. Daily morphological assessment showed that, began proliferate increase size, while viability decrease 3 cultivation, they less than 20% 21 days. went into apoptosis, their level decreased 70% control group (without feeder-cells) cultivation. A comparative evaluation different obtaining carried out. It shown when isolated by magnetic selection, proportion CD3-CD56+CD16+ more 90%, removal adherent it least 60%. When cultivating (after separation) together gmK562 21st day, possible up 85 times. adhesion) non-genetically modified days, 8 that supernatants collected during (both irradiated genetically K562), concentrations TNFα IFNγ increased many times relative group. optimal conditions culturing experimentally selected obtain